According to Mpumalanga Police spokesperson Brigadier Selvy Mohlala, the incident occurred on Sunday.
Local farmers spotted the suspicious bakkie on the Boschpoort road and quickly alerted the police.
Four suspects fled, but the driver, Phumlani Mice Nkosi (23), was apprehended.
Brigadier Mohlala said the cow was stolen from a nearby farm.
Nkosi is expected to appear in the Belfast Magistrate’s Court on December 4.