Gyimah Edwin of Ghana Black Stars during Ghana Black Stars Afcon Qualifies Training (Christian Thompson/BackpagePix)
The Black Stars engage Congo in a friendly in Brazzaville on Tuesday, September 1 before travelling to Kigali three days to face Amavubi of Rwanda in the Group H qualifier.
Grant has handed a maiden call-up to Italy-based Godfred Donsah, who recently joined Serie A side, Bologna.
Midfielder Andre Ayew makes a return to the team after missing out on the opening qualifier against Mauritius. Also included is Richmond Boakye Yiadom, who has been in and out of the team.
Ghana and Rwanda share top spot in Group H with three points apiece.
Full squad:
Goalkeepers: Razak Braimah (Cordoba, Spain), Fatau Dauda (AshGold), Richard Ofori (Wa All Stars)
Defenders: Harrison Afful (Columbus Crew, USA), John Boye (Sivasspor, Turkey), Jonathan Mensah (Evian, France), Jeffery Schlupp (Leicester City, England), Baba Rahman (Chelsea, England), Gyimah Edwin (Orlando Pirates, South Africa), Daniel Amartey, (FC Copenhagen, Denmark)
Midfielders: Rabiu Mohammed (Krasnodar, Russia), Emmanuel Agyemang-Badu (Udinese, Italy), Afriyie Acquah (Torino, Italy), Solomon Asante (T.P. Mazembe, DR Congo), Christian Atsu (Bournemouth, England), Andre Ayew (Swansea City, UK) Mubarak Wakaso (Rubin Kazan, Russia), Bernard Mensah (Getafe, Spain), Godfred Donsah (Bologna, Italy)
Strikers: Asamoah Gyan (SIPG Shanghai, China), Jordan Ayew (Aston Villa, England), Ebenezer Assifuah (Sion, Switzerland), David Accam (Chicago Fire, USA), Richmond Boakye Yiadom(Atalanta, Italy).