Matlou took to Twitter to vent her anger after her team Ma-Indies FC lost 1-0 to rivals Kanatla Ladies FC on Saturday.
The loss saw Indies miss out on a chance to represent Limpopo at the Sasol League National Championship next month.
The former African Footballer of the Year feels that the outcome of the match was decided before it started.
“We lost but heart breaking, the man referees r the one who killing women football in limpopo, Safa Limpopo is useless and corrupt, (sic)” Matlou wrote on her Twitter account. “Mr Patrick Seema, I wonder how do you feel after what happened today, are you proud as a Safa Limpopo referees cordinator?? (sic)”
The defender confirmed that her team will file a complaint and submit video evidence from the match.